Skip to content

Protocol Template

Downloads

Download this markdown file.

Name of the experimental protocol

Summary: 1-2 sentence summary.

Protocol information

Protocol Credits

  • Author(s)
    • Firstname Lastname, Institution
    • Firstname Lastname, Institution
    • Firstname Lastname, Institution
  • Maintainer/contact: Firstname Lastname, Institution: email
  • Last updated: Month, Year
  • Source protocols and references

DNA source material

  • Type: [Microbial] OR [Plant] OR [Animal] OR [Describe]
  • Collection source: Description of what source material is

Nanopore Sequencing

Computer and Bioinformatics

  • Analysis tools
  • Analysis difficulty: [Easier] OR [Challenging]
  • Command line needed: [Yes] OR [No]
  • GPU/Super-high accuracy basecalling required: [Yes] OR [No]
Reagents

Personal protective equipment

  • As recommended by original protocols (e.g., gloves, lab coat)

Sample collection and prep

  • Reagent or material 1
  • Reagent or material 2
  • Kit
    • Included
      • Reagent or material 1
      • Reagent or material 2
    • User provided
      • Reagent or material 1
      • Reagent or material 2

DNA extraction

  • Reagent or material 1
  • Reagent or material 2
  • Kit
    • Included
      • Reagent or material 1
      • Reagent or material 2
    • User-provided
      • Reagent or material 1
      • Reagent or material 2

DNA prep, library creation, and sequencing

  • Nanopore kit
    • Included kit reagent 1
    • Included kit reagent 2
  • User-provided reagents
    • Reagent or material 1
    • Reagent or material 2
Equipment and consumables

Lab equipment

  • Item 1
  • Item 2
  • Item 3

Consumables

  • Consumable 1
  • Consumable 2
  • Consumable 3

Nanopore sequencing equipment

  • Sequencing device: [MinION] OR [Flongle] OR [Promethion] OR [Other]

Computer equipment

  • Desktop or laptop
  • Other accessory or device
Estimated timings

Section I

Section II

Section III

Section IV

Section V

Background

This should be a short explanation of the protocol and background about the experiment.

Some sentence with a reference or term needing explanation, (1). Try it!

  1. Explanatory note link

Add some helpful videos about MinION

Additional Reading

Some reading and potentially links to references.

Section I

You can add annotations to a block (1) of text.

  1. A three-dimensional cube.
  1. Step one, add BSA.
  2. Step two, why is BSA underlined (1)?
  3. This is how you add annotations within an ordered list
  1. We added an entry to includes/glossary.md

So

  1. Another step 1
  2. Another step 2

Tip

An optional tip

Warning

An optional warning

Section II

One way to do this step (1). Try it!

  1. 🙋‍♂️ I'm an annotation!

Another way to do this step (1). Try it!

  1. 🙋‍♀️ I'm an annotation as well!

Section III

Nanopore

Remember to cite the authoritative Nanopore protocols

Section IV

Nanopore

Feel free to add explanatory details if relevant

Section V

Example data

If using example data, provide links to it and describe

Bioinformatics

Add bioinformatics explanatory details if relevant

Comments and discussion

See recent comments or start a discussion on our Slack channel.